Levich Institute Seminar Announcement, 09/12/2017
Steinman Hall, Room #312
(Chemical Engineering Conference Room)
Dr. Ben Ovryn
"Single Molecule Tracking of Tagged Glycans and Modeling of Beads-on-a-String Structures Along Membrane Nanotubes in Live Cells"
The modeling of the structure of plasma membrane bridges between cells (often called membrane nanotubes) has not received adequate treatment. Although the majority of membrane nanotubes appear as stable liquid cylinder connections between cells, we have recently observed from single molecule tracking of glycoproteins in live cells, that membrane nanotubes can manifest stable, beads-on-a-string structures. Similar behavior has also been observed for so-called ``beaded apoptopodia'' which are formed in dying cells. Therefore, modeling these nanotubes as Newtonian liquid filaments is incomplete. We present data from live cells and propose several mechanisms that could give rise to the observed beads-on-a-string in these membrane liquid bridges.
BRIEF ACADEMIC/EMPLOYMENT HISTORY
Ben Ovryn holds a B.S. (physics) from U. of Rochester; a M.A. (physics) from Stony Brook and a Ph.D. (biomedical engineering) from Case Western Reserve University. He is a Senior Research Scientist, Department of Electrical and Computer Engineering, Colorado State University, Fort Collins. He was a Senior Scientist at Scripps California (2016-2017) and an Associate Professor, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine (2003-2016). From 1987-2003, he was a lab head in optical diagnostics in the Microgravity Fluids Division at NASA, Cleveland and a Principal Researcher and Instructor at Case Western Reserve University (Departments of Mechanical and Biomedical Engineering).
RECENT RESEARCH INTERESTS
Single molecule imaging; interference optical microscopy; biophysics of membrane adhesions; tracking of glycans on live cells; three-dimensional particle tracking in microscopic fields of view.