Levich Institute Seminar Announcement, 04/09/2013
Tuesday, 04/09/2013
2:00 PM
Steinman Hall, Room #312
(Chemical Engineering Conference Room)

Professor Anubhav Tripathi
Brown University
Associate Professor of Engineering and Medical Sciences

"On Developing Microfluidic Reactors for Diagnostic Applications "

ABSTRACT


We are developing several microfluidic routes to understand complex biomedical assays. Biomolecular transport, amplification reaction and sequence specific identification define some of the key aspects of these assays. We will give some examples to illustrate these aspects: We will focus on in vitro transcription reaction (IVT) of both a long and short strand of H5 influenza on both free and immobilized DNA templates to study the role of mass transfer and steric effects. We will show that transcription efficiency can be dramatically increased with optimal solution chemistry and steric effects due to surrounding immobilized molecules are only significant in certain specific conditions. We will present a microfluidic reactor for RNA amplification that uses mobilized components of binding reactions. This provides a basis for appropriate use of mass transfer and reaction arguments in successful application of amplification microreactors. We will also present the dispersion of molecules in a microchannel as they undergo a DNA amplification reaction that is driven by temporally changing temperatures. The results of this study enhance our understanding of transport in microscale systems in diverse areas relevant to micro-reactor design and DNA amplification.

BRIEF ACADEMIC/EMPLOYMENT HISTORY

I am a faculty in the School of Engineering and the Division of Biological Sciences and Medical school (courtesy) at Brown University. My experience in research and teaching builds from nearly ten years at Brown, three years at Caliper LifeSciences (Hopkinton, MA) as a research scientist, a year at San Jose State University as a part-time instructor, two years at Massachusetts Institute of Technology (MIT) as a postdoctoral associate (with Professor Gareth McKinley, Mechanical Engineering) and five years at the Levich Institute, City University of New York as a graduate student (with Professor Andreas Acrivos, Chemical Engineering & Fluid Mechanics). At Brown, I serve in the admission committees for undergraduate, graduate and MD-PhD candidates. I also serve as a co-director for the Center of Biomedical Engineering, which is responsible for both undergraduate and graduate degrees in Biomedical Engineering.

RECENT RESEARCH INTERESTS:

My research focuses on understanding biochemical and biomolecular processes in microchip environments. Projects include: developing continuous flow DNA and RNA amplification processes, rapid identification of Influenza subtypes, fast screens for protein folding and unfolding buffers, separation of protein isoforms, developing micro-bubble shells for detecting pathological conditions, and developing nanoparticle induced pathogen lysis.



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